Cytoprotective effect of palm kernel cake phenolics against aflatoxin B1-induced cell damage and its underlying mechanism of action

Table 7 Percentage of viable, apoptotic and dead cells analysed by flow cytometry

Cells (%)	PEF (μg/ml)					Control+	S.E.M
Cells (%)	0	5	10	20	40	Control+	S.E.M
Viable	15.2^d	16.2^d	27.3^c	36.1^b	46.2^a	40.2^ab	3.2
Apoptotic	52.8^a	54.1^a	48.4^b	43.4^bc	37.1^d	41.1^cd	2.4
Dead	32.1^a	29.8^a	24.3^b	20.7^bc	16.9^d	18.8^cd	2.1

The cells were pre-treated with different concentrations of PEF for 24 h, then the media were replaced with a medium containing 5 μM of AFB1 and the cells were incubated for another 48 h
A minimum of 15,000 cells per sample was analysed by flow cytometry
Control+: 10 μM or 1.7 μg/ml gallic acid
Means with different superscripts (a,b,c,d) within a row are significantly different (p < 0.05)
PEF Phenolic-enriched fraction
S.E.M Standard error of the means

ISSN: 2662-7671