| Gene expression (Fold changes) |
---|
Items |  | PEF concentration (μg/ml) | Control+ | S.E.M |
---|
0 | 5 | 10 | 20 | 40 |
---|
Up-regulated genes |
Bcl2 | 1.0d
| +2.1cd
| +3.2c
| +4.3b
| +5.9a
| +5.6a
| 0.39 |
Down-regulated genes |
NF-kB | 1.0d
| −1.5d
| −2.6c
| −3.6b
| −4.4a
| −4.6a
| 0.36 |
TNF-α | 1.0d
| −1.2cd
| −2.1c
| −3.7b
| −5.1a
| −4.9a
| 0.41 |
IL1ß | 1.0d
| −1.1d
| −1.6c
| −2.2b
| −2.9a
| −3.2a
| 0.18 |
IL6 | 1.0d
| −1.3cd
| −1.7c
| −2.4b
| −3.5a
| −3.4a
| 0.21 |
iNOS | 1.0d
| −1.7cd
| −2.4bc
| −3.3b
| −4.8a
| −4.4a
| 0.43 |
Bax | 1.0d
| −1.8cd
| −2.7c
| −4.2b
| −5.7a
| −5.2a
| 0.47 |
- The cells were pre-treated for 24 h with different concentrations of PEF ranging from 0 to 40 μg/ml and positive control (10 μM or 1.7 μg/ml gallic acid). The media were replaced with a medium containing 5 μM of AFB1 and the cells were incubated for another 48 h
- The expression of each gene was normalised to the GAPDH and ß-actin expressions as housekeeping genes and then the result normalised to the expression of that gene in the negative control (PEF 0 μg/ml)
-
S.E.M Standard error of the means
- Means (n = 3) with different superscripts (a,b,c,d) within a row are significantly different (p < 0.05)