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Table 6 The changes in the expression of different genes in chicken hepatocytes pretreated with phenolic-enriched fraction (PEF) and exposed to AFB1

From: Cytoprotective effect of palm kernel cake phenolics against aflatoxin B1-induced cell damage and its underlying mechanism of action

 

Gene expression (Fold changes)

Items

 

PEF concentration (μg/ml)

Control+

S.E.M

0

5

10

20

40

Up-regulated genes

Bcl2

1.0d

+2.1cd

+3.2c

+4.3b

+5.9a

+5.6a

0.39

Down-regulated genes

NF-kB

1.0d

−1.5d

−2.6c

−3.6b

−4.4a

−4.6a

0.36

TNF-α

1.0d

−1.2cd

−2.1c

−3.7b

−5.1a

−4.9a

0.41

IL1ß

1.0d

−1.1d

−1.6c

−2.2b

−2.9a

−3.2a

0.18

IL6

1.0d

−1.3cd

−1.7c

−2.4b

−3.5a

−3.4a

0.21

iNOS

1.0d

−1.7cd

−2.4bc

−3.3b

−4.8a

−4.4a

0.43

Bax

1.0d

−1.8cd

−2.7c

−4.2b

−5.7a

−5.2a

0.47

  1. The cells were pre-treated for 24 h with different concentrations of PEF ranging from 0 to 40 μg/ml and positive control (10 μM or 1.7 μg/ml gallic acid). The media were replaced with a medium containing 5 μM of AFB1 and the cells were incubated for another 48 h
  2. The expression of each gene was normalised to the GAPDH and ß-actin expressions as housekeeping genes and then the result normalised to the expression of that gene in the negative control (PEF 0 μg/ml)
  3. S.E.M Standard error of the means
  4. Means (n = 3) with different superscripts (a,b,c,d) within a row are significantly different (p < 0.05)