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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Evaluation of in vitro anti-inflammatory effects of crude ginger and rosemary extracts obtained through supercritical CO2 extraction on macrophage and tumor cell line: the influence of vehicle type

Fig. 4

Effects of ginger and rosemary extracts on the viability and nitric oxide production by LPS and/or IFN-γ-stimulated J774 macrophages cells: influence of vehicle and concentration of extracts. Cells were incubated for 48 h with ginger and rosemary extracts dissolved in DMSO (a) and Pluronic-F68 (b) and stimulated with LPS/IFN-γ: LPS (1 μg/mL) plus IFN-γ (150 IU/mL). Cells were treated with extracts at the indicated concentrations. Cell viability was determined by MTT assay. The amount of NO released into the culture supernatants is expressed as nitrite. The columns represent the means ± SEM of the data from triplicate tests. * indicates data statistically significantly different in comparison with the control (no-treated cells) at p < 0.05

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