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Protective effect of diet supplemented with rice prolamin extract against DNCB-induced atopic dermatitis in BALB/c mice
- Hyun-Joong Yoon†1,
- Mi-Sun Jang†1,
- Hyun-Woo Kim2,
- Dong-Up Song1,
- Kwang-Il Nam1,
- Choon-Sang Bae1,
- Seong-Jin Kim1,
- Seung-Rock Lee1,
- Chang-Sub Ku3,
- Dong-Il Jang3 and
- Bong-Whan Ahn1Email author
© Yoon et al. 2015
Received: 23 April 2015
Accepted: 2 October 2015
Published: 14 October 2015
Rice prolamin has been reported to possess antioxidative, anti-inflammatory and immune-promoting properties. This study is aimed to examine the protective effects of dietary rice prolamin extract (RPE) against dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in mice.
BALB/c mice were fed diet supplemented with 0–0.1 % RPE for 6 weeks. For the last 2 weeks, 1 % or 0.2 % DNCB was applied repeatedly to the back skin of mice to induce AD-like lesions. Following AD induction, the severity of skin lesions was examined macroscopically and histologically. In addition, the serum levels of IgE, IgG1 and IgG2a were determined by ELISA, and the mRNA expression of IL-4 and IFN-γ in the skin was determined by real-time PCR.
Dietary RPE suppressed the clinical symptoms of DNCB-induced dermatitis as well as its associated histopathological changes such as epidermal hyperplasia and infiltration of mast cells and eosinophils in the dermis. RPE treatment also suppressed the DNCB-induced increase in transepidermal water loss. Dietary RPE inhibited the DNCB-induced enhancement of serum IgE and IgG1 levels, whereas it increased the serum IgG2a level in DNCB-treated mice. In addition, dietary RPE upregulated the IFN-γ mRNA expression and downregulated the IL-4 mRNA expression in the skin of DNCB-treated mice.
The above results suggest that dietary RPE exerts a protective effect against DNCB-induced AD in mice via upregulation of Th1 immunity and that RPE may be useful for the treatment of AD.
KeywordsDietary rice prolamin extract Atopic dermatitis Interferon-gamma
AD is a chronic inflammatory skin disease that often begins in infancy. It causes enormous physical discomfort and imposes huge demands on time and resources . AD is characterized by pruritus and eczematous skin lesions, elevated serum immunoglobulin (Ig) E level, and infiltration of immune cells such as mast cells, eosinophils and lymphocytes in the skin [2, 3]. Th2 cells play a key role in the pathogenesis of AD [4, 5]. They synthesize high levels of IL-4 and other Th2 cytokines, which lead to immunoglobulinemia E, eosinophilia, epidermal thickening and other AD-associated inflammatory changes. Conversely, Th1 cells suppress the Th2 immune responses through production of IFN-γ . Therefore, promotion of Th1 immunity and suppression of Th2 immunity can be an effective therapeutic measure for AD [6, 7]. In fact, some strains of probiotic bacteria have been reported to exert beneficial effects in AD by promoting IFN-γ production [8–11].
Rice is a staple food worldwide . In addition, rice has been described to have various pharmacological and biological activities including hypocholesterolemic and anticarcinogenic effects [13–20]. Rice proteins are nutritious for humans with hypoallergenic properties among the cereal proteins. They consist of four important fractions, identified by differential solubility: water-soluble albumin, salt-soluble globulin, alkali-soluble glutelin and alcohol-soluble prolamin [12, 21]. Recently, antioxidative , anti-inflammatory  and immune-promoting  activities of rice prolamin have been reported. According to Chen et al. and other investigators [24, 25], human peripheral blood mononuclear cells (HPBMCs) exposed to rice prolamin secreted IFN-γ, and the conditioned medium prepared from HPBMCs cultured in the presence of rice prolamin inhibited the growth of human leukemia U937cells and triggered the differentiation of the cells toward monocytes. Because rice prolamin is indigestible [12, 26], an exception among the rice proteins, dietary rice prolamin seems to pass through the intestine in macromolecular forms, where it may induce immune reactions. Therefore, the above observations suggest that dietary rice prolamin may be beneficial for AD via immunomodulatory function.
In the present study, we examined the protective effects of dietary supplementation of rice prolamin extract (RPE) on DNCB-induced AD-like lesions in BALB/c mice. We also examined the effects of dietary RPE on Th1 and Th2 immunities in the above mice.
Animals and materials
Six-week-old female BALB/c mice were purchased from Jungang Lab Animal, Inc. (Seoul, Korea) and were housed in an air-conditioned room (22 ± 2 °C) with a 12-h dark–light cycle and were allowed free access to water and food. 2, 4-Dinitrochlorobenzene (DNCB) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA) and dissolved in acetone-olive oil (4:1, v/v). All other reagents used were of the analytical grade commercially available. This study was approved by the Institutional Animal Care and Use Committee of Chonnam National University Medical School (approval No.: CNU IACUC-H-2015–5).
Preparation and feeding of rice prolamin extract (RPE)
Induction of AD-like lesions
The DNCB patched model described by Lee et al.  was used. The backs of mice were shaved with an electric clipper and depilatory cream a day before DNCB sensitization. The DNCB sensitization and challenge were performed for 2 weeks according to the schedule summarized in Fig. 1. For the sensitization process, a 1 cm2 gauze-attached patch (Tegaderm®, 3 M Health Care, St. Paul, MN, USA) was applied with 0.1 ml of 1 % DNCB and attached to the shaved area for 2 days on day 0 and 3. For the challenge process, the gauze-attached patch was applied with 0.1 ml of 0.2 % DNCB and attached to the sensitized area for a day on day 7 and 10. The mice were sacrificed on day 14 to evaluate the effects of DNCB and RPE treatments.
Assessment of the severity of skin lesions
The severity of DNCB-induced skin lesions was clinically assessed as previously described [29, 30]. The dermatitis score was defined as a sum of individual scores (0, no symptom; 1, mild; 2, moderate; 3, severe) for the following four signs and symptoms: erythema/hemorrhage, edema, erosion and dryness.
Measurement of transepidermal water loss (TEWL)
TEWL was measured under forane anesthesia using a Tewameter TM300 (Courage and Khazaka Electronic GmbH, Köln, Germany) in a climate-controlled room.
The dorsal skins of the mice were removed and fixed in 10 % phosphate-buffered formalin. The skin sections (4 μm thick) were stained with hematoxylin and eosin to evaluate the epidermal hyperplasia and the other sections were stained with toluidine blue and Giemsa to evaluate the infiltration of mast cells and eosinophils.
Measurement of serum immunoglobulins
Blood samples were collected by cardiac puncture under anesthesia, and sera were collected by centrifugation and stored at −80 °C until use. The serum IgE, IgG1 and IgG2a levels were measured with monoclonal antibody pairs by sandwich ELISA using commercial kits (eBioscience, San Diego, CA, USA). Briefly, microtiter plates were coated with monoclonal rat antimouse IgE, IgG1 or IgG2a antibody, followed by sequential incubation of serially diluted purified mouse IgE, IgG1 and IgG2a (standards) or sera (in triplicate), horseradish peroxidase-conjugated monoclonal antimouse IgE, IgG1 or IgG2a antibody, and then the substrate. The absorbance of the resulting product was read using a microplate reader (BioTek Instruments, Inc., Winooski, VT, USA).
Measurement of IL-4 and IFN-γ mRNA expression in the skin by real-time polymerase chain reaction (RT-PCR)
Total RNA was extracted from skin tissue with the TRIzol reagent (Invitrogen Life Technologies, Grand Island, NY, USA) according to the manufacturer’s instruction. The quantity and purity of total RNA were determined with a Nanodrop reader (Nanodrop Technologies, Wilmington, DE, USA). One microgram of total RNA was converted to the first-strand DNA with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen Life Technologies) and RNAsin (Takara, Otsu, Shiga, Japan). cDNA was amplified using gene-specific primers and GoTaq® DNA polymerase (Promega, Madison, WI, USA). Primer sequences were as follows: IFN-γ, 5′-GTCAACAACCCACAGGTCCA-3′/5′-ACTCCTTTTCCGCTTCCTGA-3′; IL-4, 5′- CTTCCAAGGTGCTTCGCATA-3′/5′-AAGCCCGAAAGAGTCTCTGC-3′; β-Actin, 5′- CTAGGCACCAGGGTGTGATG-3′/5′-GGGGTACTTCAGGGTCAGGA-3′. β-Actin was used as the internal control.
The results are presented as mean ± SD. The significance of differences of all results was analyzed by one-way analysis of variance (ANOVA) followed by the Scheffe’s test.
Effect of dietary RPE on clinical symptoms of DNCB-induced dermatitis in BALB/c mice
Histopathological changes in the skin caused by DNCB and RPE treatments
Changes in the serum concentrations of IgE, IgG1 and IgG2a caused by DNCB and RPE treatments
Effects of dietary RPE on serum levels of lgE, lgG1 and lgG2a in DNCB-treated mice
42.7 ± 14.6
0.334 ± 0.041
0.102 ± 0.019
264.0 ± 38.5**
2.326 ± 0.411**
0.110 ± 0.013
DNCB + 0.05 % RPE
175.0 ± 26.9††
1.648 ± 0.274††
0.124 ± 0.024
DNCB + 0.1 % RPE
148.7 ± 25.4††
1.388 ± 0.216††
0.159 ± 0.016†
DNCB and RPE treatments change the mRNA expression of IL-4 and IFN-γ in the skin
This study showed that dietary RPE supplementation inhibited the AD-like pathology induced by DNCB treatment in BALB/c mice. RPE treatment suppressed not only the clinical symptoms of dermatitis such as erythema, edema, erosion and dryness (Fig. 2), but also its histopathological changes such as epidermal hyperplasia and infiltration of mast cells and eosinophils in the dermis (Fig. 4). In addition, RPE treatment suppressed the DNCB-induced attenuation of skin barrier function, as evidenced by the changes in TEWL (Fig. 3). The immune cells infiltrated in the skin tissue, following antigen binding, become activated and secrete a variety of bioactive chemical mediators including histamine, proteases, eicosanoids, cytokines and chemokines, and proinflammatory proteins . Bioactive mediators can cause locally and systemically diverse symptoms and signs including those observed in Figs. 2, 3, and 4 . Therefore, our results indicate that dietary RPE may effectively prevent AD symptoms.
As shown in Table 1, dietary RPE reduced the serum levels of IgE and IgG1, whereas it raised the serum IgG2a level, in DNCB-treated mice. It is known that IFN-γ stimulates the expression of IgG2a and inhibits the production of IgE and IgG1. In contrast, IL-4 has powerful effect in stimulating the expression of IgE and IgG1 but markedly inhibits the expression of IgG2a . Although the mechanism for the anti-AD action of RPE remains unclear, the results of Table 1 strongly suggest that upregulation of Th1 immunity and downregulation of Th2 immunity are a principal mechanism for the RPE action. Indeed, we found that RPE treatment suppressed IL-4 mRNA expression and raised IFN-γ mRNA expression in the skin of DNCB-treated mice (Fig. 5), supporting the above suggestion that upregulation of Th1 immunity and downregulation of Th2 immunity are involved in the anti-AD action mechanism of dietary RPE. Because Th2 cytokine production is a default pathway in many systems [33–35] and Th1 and Th2 expressions are antagonistic to each other, it follows that upregulation of IFN-γ expression by RPE may be the primary causative factor that leads to downregulation of Th2 expression and other RPE effects observed in this study. For example, IFN-γ can seriously influence Th1/Th2 cell differentiation and cytokine production . IFN-γ activates signal transducer and activator of transcription 1 (Stat1), which upregulates the leading Th1 transcription factor, T-bet, further enhancing Th1 cytokine production. Concurrently, IFN-γ inhibits Th2 cytokine production by interfering with GATA, a Th2 transcription factor. Furthermore, inflammatory cells in local skin lesions are recruited by complex interactions of chemokines and chemokine receptors whose expression is also regulated by Th1 and Th2 cytokines [32, 37]. IFN-γ amplifies Th1 responses by inducing Th1-type chemokines and their receptors and by preventing the expression of Th2-type receptor ligands. AD is characterized by hyperactivated Th2 cytokines that lead to immunoglobulinemia E, eosinophilia, epidermal thickening and other AD-associated inflammatory changes [4, 5]. These Th2 immune responses, however, can be suppressed by IFN-γ . The formation and maintenance of skin barrier are also influenced by Th1 and Th2 cytokines . IFN-γ stimulates ceramide synthesis through the action of enzymes, sphingomyelinase and glucocebrosidase, resulting in suppression of TEWL [38, 39]. And IFN-γ may play a role in maintaining the skin barrier by regulating Th2 cytokine receptors, because Th2 cytokines inhibit the formation of skin barrier . Thus, IFN-γ can modify all the DNCB-induced AD-like changes observed in this study: clinical symptoms, attenuation of skin barrier function, immune cell infiltration, and changes in serum IgE, IgG1 and IgG2a levels and skin Th1 and Th2 cytokine mRNA expressions.
Although earlier studies showed that PBMCs exposed to rice prolamin secreted IFN-γ in vitro [27, 28], it is uncertain how dietary RPE can activate Th1 responses in vivo. As dendritic cells are the only antigen presenting cells known to sample luminal contents from the intestine [35, 40], they play a critical role in Th1 cell activation by commensal bacteria- and food-associated antigens . They sample and uptake antigens from the gastrointestinal luminal compartment and processed the antigens [40–42]. After migrating to nearby lymph nodes, they present the processed antigens to T cells and stimulate T cell differentiation. They also secrete IL-12, which binds to the IL-12 receptor on T cells and signals to activate Th1 cell differentiation . The differentiated Th1 cells can then migrate to extraintestinal sites . It will be an important task to find out whether dietary RPE acts in a similar way to the above mentioned antigens or if it acts by other mechanisms.
In the present study, dietary RPE significantly prevented the AD-like symptoms induced by DNCB treatment in mice and it improved the Th1/Th2 balance that was skewed to Th2 by DNCB treatment. Because rice is a common, stable and safe food, RPE can be a potential resource for the development of new therapeutic agents for AD. Further studies including clinical researches are required to prove this possibility.
This study indicated that dietary RPE was protective against DNCB-induced AD-like lesions in BALB/c mice. Our results suggest that dietary RPE exerts its anti-AD effect via upregulation of Th1 immunity and that RPE may be useful for the treatment of AD.
This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (Grant No.: HN11C0047).
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