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Table 6 Antibacterial activity of leaf, stem and fruit extracts of D. innoxia

From: Extraction optimization of medicinally important metabolites from Datura innoxia Mill.: an in vitro biological and phytochemical investigation

Extracts aDiameter of growth inhibition zone (mm ± SD)
K. pneumoniae MIC (μg/ml) S. typhimurium MIC (μg/ml) M. luteus MIC (μg/ml) S. aureus MIC (μg/ml)
Leaf
 Nh 8.5 ± 0.76d -- 10 ± 2.85b 33.33 7 ± 1.84d -- 9 ± 1.89c --
 C 10 ± 0.96c 33.33 6.5 ± 1.32b -- 9 ± 1.45d -- 9 ± 2.31c --
 A 10 ± 0.76c 33.33 7.5 ± 1.33d -- 14.5 ± 1.1b 3.70 10 ± 1.63b 100
 EthA 10 ± 0.98c 100 7 ± 1.54d -- 12 ± 1.17c 33.33 9 ± 1.78c --
 Eth 12 ± 0.22c 100 8 ± 1.24c -- 18 ± 1.34b 3.70 8 ± 1.49d --
 EC 10 ± 0.48c 100 7.5 ± 1.34d -- 12 ± 1.22b 33.33 8.5 ± 1.87d --
 MC 7 ± 0.45d -- 6 ± 2.21d -- 16 ± 1.45b 3.70 6 ± 1.89d --
 EthE 12 ± 0.18b 33.33 7.5 ± 1.76d -- 14 ± 1.45c 33.33 7 ± 2.21d --
 Meth 9.5 ± 0.34c -- 7 ± 1.32d -- 10 ± 1.34c 100.00 8. 5 ± 1.38d --
 E 8 ± 0.67d -- 17 ± 1.45d 11.11 13 ± 1.45c 11.11 9.5 ± 1.22b --
 M 7 ± 0.34d -- 8 ± 1.24c -- 16 ± 1.67b 3.70 7 ± 1.23d --
 D 7 ± 0.56d -- 7 ± 1.23d -- 14 ± 1.13c 11.11 6 ± 1.43d --
Stem
 Nh -- -- 10 ± 1.67c 33.33 8 ± 2.34c -- 10 ± 1.83b 100
 C -- -- 14 ± 0.88b 11.11 8 ± 1.45c -- 9 ± 1.21c --
 A -- -- 7 ± 1.21d -- 8 ± 2.2b -- -- --
 EthA -- -- 10 ± 2.67c 100 7 ± 1.22d -- -- --
 Eth -- -- 7 ± 2.34d -- -- -- -- --
 EC -- -- 7 ± 1.23d -- 13 ± 1.3b 100 -- --
 MC 9 ± 1.87c -- 9 ± 1.56c -- -- -- 7 ± 2.21d --
 EthE 8 ± 2.14c -- --   7 ± 1.45d -- -- --
 Meth -- -- --   7 ± 0.77d -- -- --
 E -- -- 10 ± 2.13c 100 20 ± 1.82c 3.70 -- --
 M 9 ± 2.32c -- 6 ± 1.43d -- 7 ± 1.23d -- 8 ± 1.21c --
 D -- -- 10 ± 1.56c 100 0 -- -- --
Fruit
 Nh -- -- 12 ± 1.21b 100 24 ± 1.32b 3.70 -- --
 C 8 ± 1.23c -- 8 ± 2.45d -- 9 ± 1.22c -- 9 ± 1.32d --
 A 8 ± 1.45c -- 7 ± 1.13d -- 8 ± 1.45d -- -- --
 EthA -- -- 13 ± 2.21b 33.33 10 ± 3.12c 100 10 ± 1.21c 100
 EC -- -- 8 ± 1.56d -- -- -- -- --
 MC -- -- 10 ± 1.56c 100 9 ± 1.82c -- -- --
 EthE -- -- 11 ± 1.65c 100 8 ± 1.21d -- -- --
 Meth 9 ± 1.76c -- 10 ± 1.67c 100 8 ± 2.13d -- -- --
 E 10 ± 1.12b 100 12 ± 2.21b 100 8 ± 1.23d -- 12 ± 1.28b 33.33
 M 7 ± 1.23d -- 12 ± 1.54b 33.33 9 ± 1.51b -- -- --
 D -- -- -- -- 10 ± 1.21c 100 -- --
 DMSO -- -- -- -- -- -- -- --
 Cefixime 28 ± 0.07 3.33 26 ± 0.21 3.33 18 ± 0.44 1.11 16 ± 1.22 1.11
  1. aZone of inhibition including the diameter of disc (5 mm). In each disc, the sample size was 100 μg per disc (5 μl) in disc diffusion assay. Values (mean ± SD) are average of three samples of each plant extract, analyzed individually in triplicate (n = 1 × 3). b-d Means difference is highly significant, slightly significant, and significant at p < 0.05. --: No activity in disc diffusion assay or not active (zone ≥ 10 mm) for MIC determination