Fig. 2

XNJ inhibits p53 expression in serum-free condition PC12 cells. PC12 cells were treated with serum or serum-free condition for 48 h in the absence or presence of XNJ. a p53 mRNA was down-regulated by XNJ. The levels of p53 mRNA were analyzed by Q-PCR. b p53 expression was down-regulated by XNJ. After XNJ treatment, the cells were lysed for Western blot analysis using an enhanced chemiluminescence system. c The density of p53/β-actin band among the experimental groups was compared. Data are mean ± SEM of values obtained from three independent experiments. *, p < 0.05 compared with serum-free condition