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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity

Fig. 2

Effect of IFA on lysine/MG-induced DNA strand breakage in the absence or presence of Cu2+. Gel images express the intensity of open circular (OC) and supercoiled (SC) forms of plasmid DNA after pUC19 (0.25 μg) was incubated with the following treatments: (a) 50 mM lysine, 50 mM MG, 300 μM CuSO4 or IFA (0.1 and 1 mM). (b) Lysine and MG in the absence or presence of various IFA concentrations (0.1–1 mM) without addition of CuSO4. (c) Lysine and MG in the absence or presence of various IFA concentrations (0.1–1 mM) co-incubating with CuSO4. The percentage of OC (% OC, see calculation method in Methods section) in each treatment was subtracted by % OC of untreated DNA before expressing in (d) and (e) which represents data from gel (b) and (c), respectively. The results are presented as mean ± SEM (n = 3). a p <0.05 when compared to lysine/MG; b p <0.05 when compared to lysine/MG/Cu2+

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