Table 1 Real-time RT-PCR oligonucleotide primers
From: The anti-inflammatory activities of ethanol extract from Dan-Lou prescription in vivo and in vitro
Gene | Primer | Sequence (5′–3′) | PCR product (bp) |
|---|---|---|---|
β-actin | Forward | TGTTACCAACTGGGACGACA | 165 |
(NM_007393.3) | Reverse | GGGGTGTTGAAGGTCTCAAA | Â |
 iNOS | Forward | CACCTTGGAGTTCACCCAGT | 170 |
(NM_010927.3) | Reverse | ACCACTCGTACTTGGGATGC | Â |
 COX-2 | Forward | TGAGTACCGCAAACGCTTCTC | 151 |
(NM_011198.3) | Reverse | TGGACGAGGTTTTTCCACCAG | Â |
 IL-6 | Forward | TCCAGTTGCCTTCTTGGGAC | 140 |
(NM_031168.1) | Reverse | GTGTAATTAAGCCTCCGACTTG | Â |