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Table 2 Names, accession number and primer sequences used in the study

From: Edible Bird’s Nest attenuates high fat diet-induced oxidative stress and inflammation via regulation of hepatic antioxidant and inflammatory genes

Name

Left sequence

Right sequence

nfkb1

AGGTGACACTATAGAATACACTCCATATTTAATGCAGA

GTACGACTCACTATAGGGAGAAATCCTCTCTGTTTCG

B2ma

AGGTGACACTATAGAATAATGCTTGCAGAGTTAAACA

GTACGACTCACTATAGGGATGCATAAAATATTTAAGGTAAGA

Hprt1a,b

AGGTGACACTATAGAATATCCTCATGGACTGATTATG

GTACGACTCACTATAGGGACTGGTCATTACAGTAGCTCTT

Rpl13aa

AGGTGACACTATAGAATAATGGGATCCCTCCAC

GTACGACTCACTATAGGGAATTTTCTTCTCCACATTCTT

Kan(r)c

  

Sod1

AGGTGACACTATAGAATAATATGGGGACAATACACAA

GTACGACTCACTATAGGGATCCAACATGCCTCTCT

Sod2

AGGTGACACTATAGAATACAGGTTGCTCTTCAGC

GTACGACTCACTATAGGGAAACTCTCCTTTGGGTTCT

Tnf

AGGTGACACTATAGAATACCCAACAAGGAGGAGA

GTACGACTCACTATAGGGATGGTGGTTTGCTACGA

CcL2

AGGTGACACTATAGAATAATAAAATTGCATCAACCCTA

GTACGACTCACTATAGGGAATCACATTCCAAATCACACT

IL6

AGGTGACACTATAGAATAATTGTATGAACAGCGATGA

GTACGACTCACTATAGGGAAGTTGTTCTTCACAAACTCC

Gpx1

AGGTGACACTATAGAATATTGAGAAGTTCCTGGTAGGT

GTACGACTCACTATAGGGATTTTCTGGAAATCAGGTGT

IL10

AGGTGACACTATAGAATAAGGACTTTAAGGGTTACTTG

GTACGACTCACTATAGGGAGAAGATGTCAAACTCATTCA

Gsr

AGGTGACACTATAGAATAAATAAACTGGGGATTCAGAC

GTACGACTCACTATAGGGAAGTAGATTTTCACATTGTCTTTG

CRP

AGGTGACACTATAGAATACTAAACAGGCCTTCGTATT

GTACGACTCACTATAGGGACAAGCCAAAGCTCTACAAT

  1. aHousekeeping genes. bNormalization gene. Underlined sequences are left and right universal left and right sequences (tags). cinternal control supplied by Beckman Coulter Inc (Miami, FL, USA) as part of the GeXP kit. RT conditions were: 48 °C for 1 min; 37 °C for 5 min; 42 °C for 60 min; 95 °C for 5 min, then hold at 4 °C. PCR conditions were initial denaturation at 95 °C for 10 min, followed by two-step cycles of 94 °C for 30 s and 55 °C for 30 s, ending in a single extension cycle of 68 °C for 1 min. B2m: Beta-2-Microglobulin; Ccl2: Chemokine (C-C Motif) Ligand 2; CRP: C-reactive protein; Gpx1: glutathione peroxidase 1; Gsr: glutathione reductase; Hprt1: Hypoxanthine Phosphoribosyltransferase 1; IL6: interleukin 6;  IL10: interleukin 10; KanR: Kanamycin resistant; nfkb1: nuclear factor Kappa B 1; Rpl13a: Ribosomal Protein L13a; Sod:superoxide dismutase; Tnf: tumor necrosis factor