Fig. 5From: Radix Scrophulariae extracts (harpagoside) suppresses hypoxia-induced microglial activation and neurotoxicityHarpagoside protect against hypoxia-induced toxicity on microglial cells through HIF-α pathway: Western blot quantification. Under 3 h’ hypoxic stimulation, the p65 content within nucleus significantly increased; while the cytosol IκB-α content decreased; theses effect can be reversed by 1 h’s pre-incubation of 10−8 M harpagoside. For the HIF-α, hypoxic stimulation can significantly enhance the nuclear HIF-α presentation; while there was no significant effect on cytosol HIF-α; theses effect can be reversed by 1 h’s pre-incubation of 10−8 M harpagoside. Intensity of individual bands was quantified using NIH ImageJ densitometry software, and expressed relative to β-actin/histone (H3) signal, as a measure of protein relative abundance in the different samplesBack to article page