Skip to main content

Advertisement

Table 2 Cytotoxicity of extracts and compounds from Nauclea pobeguinii towards sensitive and drug-resistant cancer cell lines and normal cells as determined by the resazurin assay

From: Cytotoxicity of selected Cameroonian medicinal plants and Nauclea pobeguinii towards multi-factorial drug-resistant cancer cells

Cell lines Tested samples, IC50 values and degrees of resistance (in bracket)
Crude extracts and IC50 values (μg/mL) IC50 values of compounds (μM) IC50 values of doxorubicin (μM)
NPB NPL 1 2 3 4 5 6
CCRF-CEM 14.62 ± 1.23 25.84 ± 2.16 >82.92 >243.90 >170.94 28.15 ± 1.32 25.08 ± 2.48 77.10 ± 5.87 0.20 ± 0.06
CEM/ADR5000 11.56 ± 0.85 (0.80) 25.55 ± 1.63 (0.99) >82.92 (n.a) >243.90 (n.a) >170.94 (n.a) 57.43 ± 3.67 (2.04) 39.87 ± 2.91 (1.59) >80.29 (>1.04) 195.12 ± 14.30 (975.60)
MDA-MB231 37.00 ± 2.91 64.75 ± 5.08 >82.92 >243.90 >170.94 19.90 ± 1.45 97.64 ± 7.60 >80.29 1.10 ± 0.28
MDA-MB231/BCRP 19.79 ± 2.13 (0.53) 59.55 ± 4.21 (0.92) >82.92 (n.a) >243.90 (n.a) >170.94 (n.a) 22.93 ± 1.64 (1.15) 95.59 ± 8.17 (0.98) 78.26 ± 6.22 (<0.97) 7.83 ± 0.47 (7.12)
HCT116 (p53+/+) 16.19 ± 1.16 32.78 ± 2.67 >82.92 >243.90 >170.94 73.65 ± 5.67 63.77 ± 4.61 >80.29 1.41 ± 0.29
HCT116 (p53−/−) 8.70 ± 0.68 (0.54) 19.39 ± 1.34 (0.59) >82.92 (n.a) >243.90 (n.a) >170.94 (n.a) >175.36 (>2.38) 47.03 ± 4.94 (0.74) >80.29 (n.a) 4.06 ± 0.07 (2.88)
U87MG 69.44 ± 4.34 >40 >82.92 >243.90 >170.94 76.59 ± 4.92 55.64 ± 3.73 >80.29 1.06 ± 0.15
U87MG.ΔEGFR 32.78 ± 2.79 (0.47) 63.90 ± 4.77 (>1.60) >82.92 (n.a) >243.90 (n.a) >170.94 (n.a) 28.45 ± 2.06 (0.37) 47.59 ± 3.29 (0.86) >80.29 (n.a) 6.11 ± 0.57(5.76)
AML12 >40 >40 >82.92 >243.90 >170.94 >175.36 >102.56 >80.29 >73.59
  1. (*): The degree of resistance was determined as the ratio of IC50 value in the resistant divided by the IC50 in the sensitive cell line; NPB: extract from the bark of Nauclea pobeguinii; NPL: extract from the leaves of Nauclea pobeguinii; Compounds isolated from NPB [1: 3-acetoxy-11-oxo-urs-12-ene; 2: p-coumaric acid; 3: citric acid trimethyl ester; 4: resveratrol; 5: resveratrol β-D-glucopyranoside; 6: strictosamide]; n.a.: not applicable. IC50 value are mean ± SD of at least two experiments with six replicates each