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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Induction of substantial myocardial regeneration by an active fraction of the Chinese herb Rosa laevigata Michx

Fig. 5

Morphological assessment of the therapeutic effect of aFRLM on MI. Representative micrographs of the aFRLM-treated (4 weeks) MI heart (Tr-1) showed that many red-stained myocyte-like cell clusters (blue rectangular surrounding) in the central area of the infarct were found that replaced the infarcted cardiac tissues. In contrast, the large area of fibrous scar was found throughout the entire infarct region (Fib) in the vehicle-treated MI heart (Ct-1) with few myocyte-like cell clusters. Masson’s trichrome staining demonstrated that although the infarcted cardiac tissues were replaced by blue-stained fibrous scar tissues, many red-stained myocyte-like cell clusters were found in the central infarct region (tr-2). However, in the vehicle-treated MI heart (Ct-2), the blue-stained fibrous scar replacement of the infarcted cardiac tissues was found throughout the entire infarcted region with few red-stained myocyte-like cells clusters. Some of the myocyte-like cells in the central infarct (blue arrows) were positively stained by both Ki-67 (brown nuclei)- and MHC (yellow cytoplasm)-specific antibodies in the aFRLM-treated MI hearts (Tr-3). Some of the regenerating myocytes joined together in tandem, forming a myocardium-like tissue. In contrast, fibrous scar replacement was found throughout the infarct region (Fib) with few myocyte-like cells that stained positively for both Ki-67 and MHC (Ct-3)

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