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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Retino-protective effect of Bucida buceras against oxidative stress induced by H2O2 in human retinal pigment epithelial cells line

Fig. 5

Cytotoxic action of hydrogen peroxide in ARPE-19 cells. A range of concentrations of H2O2 from 12.5 to 1600 μM was added to serum-containing DMEM. Cytotoxicity was estimated 24 h after addition by the MTT assay in cultures in which the medium was not replaced (solid bars) or was replaced at 4 h with fresh H2O2-free DMEM (hatched bars) or was measured at 4 h (gray-hatched bars). Addition of catalase 200 U/ml to the 1600 μM H2O2 as CAT control cells. * IC50 in 24 h ** IC50 4 h with replace (4 h + 20 h). Data expressed as a percentage of the untreated control, are from three independent experiments and are the means ± SD

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