Fig. 16

Caspase-3 activity induced by H₂O₂ addition (800 or 1600 μM) and GOx (25 or 50 mU/ml) in ARPE-19 cells for 24 h. Control, C⁺ cells were untreated with H₂O, C⁺ (the specific caspase-3 control). Cells were co-treated with 1600 μg/ml of Bucida buceras extract. Control GOx + CAT (50 mU/ml + catalase 200 U/ml) or control cells (untreated with H₂O₂). Values are presented as means ± SD of at least 3 independent experiments. *p < 0.05 significantly different from value of control vs. Control GOx + CAT (50 mU/ml + catalase 200 U/ml) or control cells (untreated with H₂O₂) Bucida buceras, or H₂O₂-treated only; **p < 0.05 significantly different from value of antioxidant treatment vs. H₂O₂-treated alone