Fig. 1

Calibration curves for H₂O₂ levels in the range 10–200 μM using ferrous-xylenol orange with 10 % FBS (D10; linear regression y = 0.0022x + 0.0149, R² = 0.98616 and without FBS (DMEM linear egression: y = 0.0025x + 0.0157, R² = 0.98643. Therefore H₂O₂determination was measured in PBS. Data are from three independent experiments and are the means ± SD of three culture wells per group in each experiment