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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Ethanol extract of propolis protects macrophages from oxidized low density lipoprotein-induced apoptosis by inhibiting CD36 expression and endoplasmic reticulum stress-C/EBP homologous protein pathway

Fig. 3

Effect of EEP on ox-LDL- or TM-induced apoptosis in RAW264.7 cells. a After RAW264.7 cells were treated with EEP (7.5, 15 and 30 mg/L) or PBA (5 mmol/L) in the presence of ox-LDL (100 mg/L) treatment for 24 h, cell apoptosis was detected using flow cytometry and the total apoptotic cells (early and late-stage apoptosis) were represented by the right side of the panel (Annexin V staining alone or together with PI). b and c Cells were pretreated with EEP (30 mg/L) or PBA (5 mmol/L) for 1 h, followed by exposure to ox-LDL (100 mg/L) for 24 h or TM (5 mg/L) for 12 h, and then cell apoptosis was detected by TUNEL assay. Scale bar =20 μm. d and e Cells were pretreated with the indicated concentrations of EEP or PBA (5 mmol/L) in the presence of ox-LDL (100 mg/L) for 24 h or TM (5 mg/L) treatment for 12 h, and then caspase-3 activity was determined by colorimetric assay. Data are expressed as the mean ± SEM of at least four independent experiments. *P < 0.05, **P < 0.01 versus vehicle-treated control; #P < 0.05, ##P < 0.01 versus ox-LDL or TM treatment

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