Fig. 1From: Immunomodulatory effects of preparations from Anthroposophical Medicine for parenteral useEffects of injectable preparations on apoptosis and necrosis of lymphocytes and DCs. After stimulation of lymphocytes (a) or DCs (b) with antihuman CD3/28 mAbs (each 10 ng/mL) or TNF-alpha, LPS and IL-1beta, respectively, in the presence of medium, camptothecin (100 μM), Triton X-100 (0.5 %) or different concentrations of Bryophyllum, Colchicum and Mandragora (all 1:300 (only in A); 1:100; 1:30; 1:10) or Rosmarinus (1:400 (only in A); 1:200; 1:100; 1:50). The cells were stained with annexin V and propidium iodide (PI) to assess the percentage of apoptotic (annexin V+/PI−) and (annexin V+/PI+) or necrotic (annexin V−/PI+) cells. The cells were analysed by flow cytometry. The results from two to three independent experiments are summarized, and data are presented as mean ± standard deviation (SD)Back to article page