Fig. 6

Protective effects of PMC-12 on regulation of intracellular protein kinases in HT22 cells. Cells were pretreated with 1 μg/ml PMC-12 or 1 mM NAC, followed by exposure to 5 mM glutamate (a). In addition, cells were pretreated with 10 μM BAPTA-AM or NAC for 30 min before addition of PMC-12 or glutamate (b). The histogram for Fig. 6b was indicated as the mean ± SEM of three independent experiments (c). Equal amounts of proteins and each sample were subjected to Western blot analysis using the indicated antibodies. Equal protein loading was confirmed by actin expression. ^###P < 0.001 vs. normal control^; ***P < 0.001 vs. glutamate-treated groups; ^$P < 0.05 vs. groups except control and glutamate-treated groups