Skip to main content
Fig. 8 | BMC Complementary and Alternative Medicine

Fig. 8

From: The flavonoid beverage Haelan 951 induces growth arrest and apoptosis in pancreatic carcinoma cell lines in vitro

Fig. 8

Effect of calpain inhibition and cytosolic Ca2+ binding on the proliferation of Haelan-treated carcinoma cells and fibroblasts. CAPAN-1 cells (a), BxPC-3 cells (b), AR42J cells (c) and fibroblasts (d) were incubated with 8 % Haelan or vehicle in the presence or absence of the calpain inhibitor PD150606 or the Ca2+ chelator BAPTA-AM. After 24 h, the proliferation was measured using the WST-1 assay. Inhibition of calpain activity or Ca2+ chelation induces an additional decrease in the proliferation of Haelan-treated CAPAN-1 and BxPC3 cells, but not in the AR42J cells. In fibroblasts, Haelan fails to induce growth arrest, whereas calpain inhibition or binding of Ca2+ initiates this mechanism. The results are expressed as mean + SEM (n = 6-12). *P < 0.05, ***P < 0.001, control vs. Haelan-treated cells, xP < 0.05, xxP < 0.01, xxxP < 0.001, Haelan-treated cells vs. cells treated with Haelan in the presence of PD150606 or BAPTA-AM

Back to article page