Fig. 7
Effect of calpain inhibition and cytosolic Ca2+ binding on the viability of Haelan-treated pancreatic carcinoma cells and fibroblasts. CAPAN-1 cells a, BxPC-3 cells b, AR42J cells c and fibroblasts d were incubated with 8 % Haelan or vehicle in the presence or absence of the calpain inhibitor PD150606 or the Ca2+ chelator BAPTA-AM. After 24 h, the cellular viability was assessed using the LDH cytotoxicity assay. Inhibition of calpain activity or Ca2+ chelation has no effect on the viability of Haelan-treated pancreatic carcinoma cells and fibroblasts. The results are expressed as mean + SEM (n = 3-7)