Fig 5

Effect of XYXD on fibrogenic response induced by LPS plus TGF-β1 in HSC. Col 1(α1) (a), TIMP1 (b) was measure by real-time PCR analysis. GFP-Col-HSC cells were pretreated with XYXD (5 μg/ml and 25 μg/ml) for 1 h and subsequently treated with LPS for 12 h, then treated with TGF-β1 (10 ng/ml) for 24 h . Data represent the mean ± SD of triplicate cultures. *P < 0.05, * *P < 0.01, vs no LPS/TGF-β1, ^#P < 0.05, ^##P < 0.01, vs vehicle. A representative result is shown. Similar results were obtained in three independent experiments