Fig. 4

Effect of XYXD on TGF-β1-induced signaling in HSC. a, NF-κB luciferase was measured. GFP-Col-HSC cells were pretreated with XYXD (5 μg/ml and 25 μg/ml) for 1 h and subsequently treated with TGF-β1 (10 ng/ml) over night, followed by transfect NF-κB-luciferase reporter plasmid for 16 h. *P < 0.05, * *P < 0.01, vs Un-TGF-β1 and No XYXD, ^#P < 0.05, ^##P < 0.01, vs TGF-β1 and No XYXD. b, western blots analysis were analysis for p-Smad2, total smad2, p-smad3, and total Smad3. GFP-Col-HSC cells were pretreated with XYXD (5 μg/ml and 25 μg/ml) for 1 h and subsequently treated with TGF-β1 (10 ng/ml) for 30 min. c, the Col1(α1) and TIMP1was measure by real-time PCR. GFP-Col-HSC cells were pretreated with XYXD (5 μg/ml and 25 μg/ml) for 1 h and subsequently treated with TGF-β1 (10 ng/ml) for 24 h. Data represent the mean ± SD of triplicate cultures. *P < 0.05, * *P < 0.01, vs vehicle, ^#P < 0.05, ^##P < 0.01, vs TGF-β1. A representative result is shown. Similar results were obtained in three independent experiments