Table 1 Progression toward clonic-like movement with PTZ in adult zebrafish

1

Stay or move near the bottom of the tank

0.10 mg/ml (0.72 mM)

2

Rapid movement in all directions,

0.10 mg/ml (0.72 mM)

3

Hit the tank walls

0.50 mg/ml (3.6 mM)

4

Rapidly go to the water surface and then rapidly go to the bottom of the tank

0.70 mg/ml (5.0 mM)

5

Shaking movement

^a

6

Erratic movements (circular motion, diagonal movement)

0.70 mg/ml (5.0 mM)

7

Wild jumping, Clonic-like movement (convulsion) then loss of coordination/ posture, remaining at the bottom

0.70 mg/ml (5.0 mM)

8

Recover posture/motion

0.70 mg/ml (5.0 mM)

9

Death

1 mg/ml^b (7.5 mM)