Water extract of brewers’ rice induces apoptosis in human colorectal cancer cells via activation of caspase-3 and caspase-8 and downregulates the Wnt/β-catenin downstream signaling pathway in brewers’ rice-treated rats with azoxymethane-induced colon carcinogenesis

Table 1 Nucleotide sequence of PCR primers for amplification and sequence-specific detection of cDNA (obtained from GenBank database)

Primer name [accession number]	Oligonucleotides (5′-3′)
Primer name [accession number]	Sequence
AXIN1 [XM_005255610.2]	F- TTTCACCGAAGATGCTCCCC
AXIN1 [XM_005255610.2]	R- CACTGCCCTCAGGCTCATAC
CK1 [NM_001271741.1]	F- GAGATCCCTTTCCCAGAGTGC
CK1 [NM_001271741.1]	R- TTTGTGAAGGGCTTCTCGGC
GSK3β [BC012760]	F- CGAATGGGGAACAGTCGAGG
GSK3β [BC012760]	R- TCGGAAATGCGACGGGAAAC
APC [NM_000038.5]	F- AGCAAGTTGAGGCACTGAAGA
APC [NM_000038.5]	R- TCCCGGCTTCCATAAGAACG
LRP6 [NM_002336.2]	F- ACATGACAGGTCGAGAGGGT
LRP6 [NM_002336.2]	R- CCAAGCCACAGGGATACAGT
ACTB^a [NM_001101.3]	F- AGAGCTACGAGCTGCCTGAC
ACTB^a [NM_001101.3]	R- AGCACTGTGTTGGCGTACAG
GAPDH^a [NM_002046.4]	F- GGATTTGGTCGTATTGGGC
GAPDH^a [NM_002046.4]	R- TGGAAGATGGTGATGGGATT
18S rRNA^a [HQ387008.1]	F- GTAACCCGTTGAACCCCATT
18S rRNA^a [HQ387008.1]	R-CCATCCAATCGGTAGTAGCG

ACTB- beta-actin, APC- adenomatous polyposis coli, CK1- casein kinase 1, GAPDH- glyceraldehyde-3-phosphate dehydrogenase, GSK3β- glycogen synthase kinase 3β, LRP6- low density lipoprotein receptor-related protein 6
^aHousekeeping gene

ISSN: 2662-7671