Fig. 3

CBF changed the distribution of cortactin in HCT116 cells (1 % oxygen). a Subcellular protein extraction of four fractions, which are cytosolic, membrane/organelle, nucleic and cytoskeletal fractions. Cortactin expression was diminished significantly in the fraction of cytoskeletal proteins after exposure to 1 μM of CBF. b Co-localisation of cortactin in treated HCT116 cells. Under a hypoxic condition, HCT116 cells were incubated with 1 μM CBF for 24 h. The subsequent staining revealed a colour overlapping, indicating that CBF induced a nuclear translocation of cortactin. Scale bars equal 10 μm