Fig. 4

Ultrastructural effects of AEPa on promastigotes of Leishmania (L.) amazonensis. a General view of untreated parasite showing the characteristic structure of kinetoplastids. b Promastigotes treated with 50 μg/mL AEPa for 3 days. Note some vacuoles in the membrane of flagellar pocket (*) and alterations in flagellar membrane (arrow). c and d General view of promastigotes treated with 100 μg/ml AEPa for 3 days. c Observe the presence of myelin-like structures in the flagellar pocket (arrows) and duplication of kinetoplast DNA (arrowhead). (d) Note the presence of a large number of vesicles inside the flagellar pocket (*) and alterations in shape and swelling of kinetoplast (arrowheads). N, nucleus; FP, flagellar pocket; K, kinetoplast; F, flagellum; M, mitochondria. Bars represent A 5 μm; B 2 μm; C 5 μm; C inset 2 μm; D 2 μm