Fig. 1

Effects of L-Cit on insulin signaling in H4IIE cells. H4IIE cells were treated with L-Cit for 1 h and stimulated for 10mins with 0.1nM insulin. a Phosphorylation level of Akt at serine 473, (b) PEPCK mRNA level, (c) phosphorylation level of IRS1 at tyrosine, (d) protein level of IR. Data were expressed as the mean ± SEM of three independent experiments. Significant differences were identified by Tukey’s test, #p < 0.05 vs control, *p < 0.05 vs with insulin