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Table 3 Characteristics of sequences and primers for high resolution melting analysis

From: Fast and reliable detection of toxic Crotalaria spectabilis Roth. in Thunbergia laurifolia Lindl. herbal products using DNA barcoding coupled with HRM analysis

Regions mat K rbc L rpoC trn L
Available species 5 6 5 19
Variable characters (%) 30.67 10.07 10.19 8.90
Conserved forward primer/total (%) 15/26 (57.69) 21/22 (95.45) 18/21 (85.71) 19/21 (90.48)
Conserved reverse primer/total (%) 19/23 (82.61) 25/25 (100) 19/21 (90.48) 24/27 (88.89)
Average %GC content 35.20 46.60 44.26 34.50