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Table 3 Characteristics of sequences and primers for high resolution melting analysis

From: Fast and reliable detection of toxic Crotalaria spectabilis Roth. in Thunbergia laurifolia Lindl. herbal products using DNA barcoding coupled with HRM analysis

Regions

mat K

rbc L

rpoC

trn L

Available species

5

6

5

19

Variable characters (%)

30.67

10.07

10.19

8.90

Conserved forward primer/total (%)

15/26 (57.69)

21/22 (95.45)

18/21 (85.71)

19/21 (90.48)

Conserved reverse primer/total (%)

19/23 (82.61)

25/25 (100)

19/21 (90.48)

24/27 (88.89)

Average %GC content

35.20

46.60

44.26

34.50