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Figure 3 | BMC Complementary and Alternative Medicine

Figure 3

From: Identification of anti-inflammatory fractions of Geranium wilfordii using tumor necrosis factor-alpha as a drug target on Herbochip® – an array-based high throughput screening platform

Figure 3

Binding signals of GWE-herbochip probed by β-TNF-α. (A) The image was visualized by Cy5-labeled streptavidin after the binding of β-TNF-α to the GWE-herbochip as described in Methods. (B) The fluorescence intensity of the image was quantized by a scanner at 635 nm presented (───) together with the original HPLC profile monitored at 254 nm (− − −−). The controls spots in (A) are 4, 10, 50, 250 ng/mL biotin in Optifix I, 1 μg/mL SA-Cy5 in Optifix II (positive control) and Optifix I (negative control) were spotted onto the slide in the same format shown in Figure 1. Positive signals indicated binding activity of the fractions to TNF-α.

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