Figure 1

Schematic representation of the modified Kupchan partitioning of methanol crude extracts of T. orientalis, B. monnieri, C. frutescens, B. oleracea and U. sinuata. The crude extract was (5 g) was triturated by dissolved in 10% aqueous methanol (methanol: water; 9:1 v/v) to make the mother solution which was partitioned off successively by four solvents such as n-hexane (HX: 820 mg), chloroform (CH: 550 mg), ethyl acetate (EAT: 665 mg) and hydro-methanol (HM: 1.5 g) in order of increasing polarity by using separating funnel.