Figure 5

Effects of SCRT on the expression of Bcl-2, Bax and cytochromec, and values of MMP in A549 cells. (A) Cells were treated with SCRT for 72 h, and aliquots containing total proteins were subjected to SDS-polyacrylamide gels followed by immunoblot analysis with specific antibodies. (B) The cytosolic and mitochondrial proteins were extracted from cells and analyzed by Western blotting using the indicated antibodies. Actin and cytochrome oxidase IV (COX IV) were used as internal controls for the cytosolic and mitochondrial fractions, respectively. The relative ratios of expression in the results of the Western blotting were presented at the bottom of each of the results as relative values of the actin or COX IV or expression. (C) The cells were collected and incubated with JC-1 (10 μM) for 20 min at 37°C in the dark. The cells were then washed once with PBS and analyzed by a flow cytometer. The data is expressed as the mean of two independent experiments.