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Figure 7 | BMC Complementary and Alternative Medicine

Figure 7

From: Concentration effects of grape seed extracts in anti-oral cancer cells involving differential apoptosis, oxidative stress, and DNA damage

Figure 7

GSE treatments caused γH2AX-based DNA damage in Ca9-22 cells. Cells were treated with different GSE concentrations for 24 h before subjected to anti-phospho-γH2AX/PI double staining for measuring DNA double strand breaks by flow cytometry.(A) A representative γH2AX/PI profile for GSE-treated cells.(B) Quantitative analysis of γH2AX-based DNA damage in GSE-treated cells. The mean fluorescence intensity of phospho-γH2AX for gated regions in (A) was calculated. Data, mean ± SD (n = 3). Treatments with the same capital letter are non-significant.

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