Figure 3

Autocorrelation curves G(τ) of fluorescence fluctuations. Representative autocorrelation curves G(τ) of fluorescence fluctuations δF(t) of TAMRA-Aβ_1–42 (5 nM) mixed with excess (20 μM) unlabeled Aβ_1–42 without (A, control) or with 20 μM asiaticoside (B). The blue lines represent the raw data, whereas the red lines represent fitted data. The curve fit was good as seen in the residuals, which are the differences between the data and the fitted curve. The residuals of a good fit fluctuate randomly and tightly about zero. (C). Diffusion times of TAMRA-Aβ_1–42 during Aβ_1–42 fibrillation in the absence (Aβ_1–42 alone, control) and presence of different concentrations of asiaticoside (5–20 μM). Results are presented as the mean ± SE of quadruplicate determinations (n = 5).