Figure 2

The cytotoxic effects of the different fractions against tumor cells. (A), Effects of different concentrations of HE, WE, CE and TAFU on proliferation of LLC, A2780, HepG2 and A549 cells. (a), HE; (b), WE; (c), CE; (d), TAFU. Three cell lines were treated with four fractions for 72 hours. Significant differences compared with Control group were designated as ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001. (B), Time and dose effects of different concentrations of TAFU on LLC, A2780, HepG2 and A549 cells growth. (a), LLC; (b), A2780; (c), HepG2; (d), A549. Significant differences of inhibitory effects between treatment of 24 h and 72 h were designated as ^*P < 0.05, ^** P < 0.01 and ^***P < 0.001; Significant differences of inhibitory effects between treatment of 24 h and 48 h were designed as ^□P < 0.05 ^□□P < 0.01 and ^□□□P < 0.001; Significant differences of inhibitory effects between treatment of 48 h and 72 h were designed as ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001. (C), Microscopic image of TAFU (IC₅₀) treated LLC, A2780, HepG2 and A549 cells for 24 and 72 hours. Cells morphology was observed by a phase contrast inverted microscope (200×). (a), LLC; (b), A2780; (c), HepG2; (d), A549. All results were expressed as the mean ± S. E. M. (n = 3).