GA inhibits cell growth in HeLa cells. (A) Formula of Gambogic acid (GA). (B) Effect of GA on cell viability in HeLa cells. Time- and dose-dependent effect of GA was performed when HeLa cells were treated with various concentrations of GA at different time points and their viability was determined by trypan blue exclusion assay. Results are mean values ± SD of three independent experiments (n = 3).