Effects of Oryeongsan on translocation of p-Smad-2 (A) and Smad-4 (B) into the cell nucleus in rat mesangial cells. Immunofluorescent images of p-Smad-2 (A) and Smad-4 (B) nuclear translocation (red arrow) under the laser scanning confocal microscopy were show (magnification. 400X). Nuclei were stained with DAPI (blue) and p-smad-2 (A) and Smad-4 (B) were stained with Alexa Fluor 488 (green). Green fluorescence indicates localization of p-Smad-2/Smad-4. Respective data were obtained from five independent experiments.