Effect of Oryeongsan on HG-induced type IV collagen and CTGF level. Cell lysates were used for Western blot analysis with a primary antibody against type IV collagen and CTGF. β-actin protein was used as an internal control. (A) Western blotting and (B) real-time PCR showed protein and mRNA levels of type IV collagen and CTGF in Oryeongsan-treated and HG-stimulated rat mesangial cells. Each value represents the means ± S.E. of five independent experiments. **p < 0.01 vs. control; #p <0.05, ##p < 0.01 vs. HG alone.