Effects of Oryeongsan on rat mesangial cell proliferation. (A) Mesangial cells were seeded into the 24-well plates. After confluence, the cells incubated for 24 h with or without HG and various concentrations of Oryeongsan, and then pulse-labeled with [3H]-thymidine for 24 h. (B) Inhibitory effect of Oryeongsan on proliferation of mesangial cells. Cells were incubated with indicated concentrations of ORS (from 0, 1, 10, and 50 μg/mL) with or without HG (25 mM) for 24 h. The proliferation inhibition was determined by used Countess™ cell counting. Results are expressed as the mean ± S.E. from five independent experiments. *p < 0.05 vs. control; #p <0.05 vs. HG alone.