Figure 4

Effect of DECPR on cell membrane permeability, MMP and cytochrome c release. (A) HT-29 cells were treated with different concentrations of DECPR for 24 h and stained with Hoechst 33342 (blue) for nucleus and phalloidin (red) for polymerized actin (F-actin). White arrows depict DNA fragmentation and shrinkage. (B) Representative bar chart showed the significant reduction in fluorescent intensity of phalloidin at 12.5 to 50 μg/mL concentrations showing the cytoskeletal rearrangement induced by DECPR. The data represent the means ± SEM of three independent experiments. *P < 0.05 compared with the untreated group.