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Table 1 Tentative assignment of HPLC chromatogram peaks as flavonoids, phenylcarboxylic acids or diterpenes based on UV absorbance and MS fragmentation patterns

From: An in vitro study of anti-inflammatory activity of standardised Andrographis paniculata extracts and pure andrographolide

Peakidentitycor number Rt (min) % Area3 Fold variation2 UV peaks1(nm) MS fragmentation (m/z)5 Tentative assignment4
Chlorogenic acid 12.2 1.0 34.3 327, 218, 235 Not determined (ND) Phenyl carboxylic acid
Isoquercetin 28.7 0.9 5.1 204, 255, 353 ND Flavonol glycoside
Peak 3 29.2 0.6 9.8 323, 219, 234 ND Phenyl carboxylic acid
Peak 4 29.7 3.5 3.8 325,219,235 ND Phenyl carboxylic acid
Peak 5 31 1.4 3.4 326, 219, 271 ND Phenyl carboxylic acid
Peak 6 32.2 4.1 6.1 324, 219, 234 ND Phenyl carboxylic acid
Peak 7 32.4 3.3 4.5 325, 220 ND Phenyl carboxylic acid
Peak 8 32.8 3.3 4.7 346, 224, 256 ND Phenyl carboxylic acid
Peak 9 33.7 2.1 4.5 204, 336, 266 ND Flavone
Peak 10 36.8 1.3 6.6 327, 219, 235 ND Phenyl carboxylic acid
Peak 11 37.7 0.8 4.4 327, 271, 223, 204 ND ND
Andrographolide 38.4 ND ND 227 351 [M+H]+, 333 [M-H2O]+, 315 [M-2H2O]+, 297 [M-3H2O]+ Diterpene
Andropanoside 39.8 1.4 4.8 207 535 [M+K]+ Diterpene
Peak 14 40.2 8.0 7.1 225 ND ND
Peak 15 40.7 4.3 3.7 202 ND ND
Apigenin 42.2 2.6 6.7 211, 337, 267 ND Flavone
Wogonoside 45.2 0.7 2.3 265, 212 ND O-methylated flavone glycoside
Peak18 46.6 0.7 4.6 ND ND ND
Peak 19 46.8 0.8 2.5 ND ND ND
Neoandrographolide 47.7 9.3 2.7 201 503 [M+Na]+,519 [M+K]+, 319[M+H-Glu]+ Diterpene
Peak 21 48.6 0.5 3.5 ND ND ND
Peak 22 49.7 1.5 5.3 ND ND ND
Peak 23 50.2 0.76 7.7 ND ND ND
Deoxyandrographolide 52.1 6.7 3.7 200 357 [M+Na]+, 317 [M+H-H2O]+, 299 [M+H-2H2O]+ Diterpene
Dehydroandrographolide 52.6 35.1 2.1 200, 249 355 [M+Na]+, 315 [M+H-H2O]+, 297 [M+H-2H2O]+ Diterpene
Peak 26 53.6 0.8 2.8 229 ND ND
Peak 27 54.3 2.0 6.1 229 ND ND
Peak 28 54.6 2.8 3.0 200, 263 ND ND
  1. 1UV peaks are listed in order of intensity.
  2. 2Fold variation = (Max. peak .area) / (Min. peak area).
  3. 3Percent (%) area = average peak area from the 12 batches / total combined area × 100. Andrographolide was excluded from the % area calculations.
  4. 4Assignments based on UV spectrum, MS fragmentation and/or comparison to reference standard RT.
  5. 5MS fragments are listed in order of intensity.