Chromatograms of standardisedA. paniculataextracts. A) The chromatograms of twelve batches (#1 at the bottom, #12 at the top) standardised to contain 30% (w/w) andrographolide were recorded at 227 nm. B) Composite chromatograms illustrating the variation between the HPLC profiles of the 12 batches. The black chromatogram was generated using the highest peaks from each of the 12 extracts. The red chromatogram was generated using the smallest peaks. Vertical blue lines indicate the retention time of the peaks that were used for the quantitative analysis of batch-to-batch variation (see Table 1). Numbered peaks were identified as chlorogenic acid (#1), isoquercetin (#2), andrographolide (#12), andropanoside (#13), apigenin (#15), wogonoside (#16), neoandrogrpapholide (#19), deoxyandrographolide (#23), and 14-deoxy-11,12-didehydroandrographolide (#24).