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Figure 8 | BMC Complementary and Alternative Medicine

Figure 8

From: Essential oils purified from Schisandrae semen inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 activation and migration of human aortic smooth muscle cells

Figure 8

Inhibition of NF-κB nuclear translocation by SSeo in TNF-α-stimulated HASMCs. (A) Cells were pretreated with SSeo (20 μg/ml) for 1 h before TNF-α treatment (100 ng/ml) for the indicated times. (A) Cytosolic and nuclear proteins were run on 10% SDS-polyacrylamide gels followed by Western blotting using anti-NF-κB p65 and anti-IκB-α antibodies, and an ECL detection system. Nucleolin and actin were used as internal controls for the nuclear and cytosolic fractions, respectively. (B) The cells were pretreated with SSeo (20 μg/ml) for 1 h before TNF-α treatment (100 ng/ml). After a 1 h incubation, localization of NF-κB p65 was visualized with fluorescence microscopy after immunofluorescence staining with anti-NF-κB p65 antibody (green). Cells were also stained with DAPI to visualize nuclei (blue). Results are representative of those obtained from three independent experiments.

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