Effect of EA pretreatment on Cx43 phosphorylation in perfused hearts subjected to SGIR. Panel A: Representative traces of electrophoresis of myocardial Cx43 protein at 41 kilo-dalton after probed with monoclonal anti-Cx43 antibody in different groups. Panel B: Group results showing the changes of relative density of myocardial nonphosphorylated Cx43 protein in different groups. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was taken as the internal control. *P < 0.05 as compared with control group; #P < 0.05 in comparison with SGIR group; +P < 0.05 in comparison with EA group (n = 20).