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Figure 6 | BMC Complementary and Alternative Medicine

Figure 6

From: Anti-angiogenic effects of pterogynidine alkaloid isolated from Alchornea glandulosa

Figure 6

Capillary-like structures in HUVEC cultures and semiquantification of the tube formation index. a) Photomicrographs of capillary-like structures assembly in HUVEC cultures after treatment with Pt. In contrast to control cells, incubation with 8 μM Pt resulted in absence of ramifications with undifferentiated cells. Photomicrographs are representative of the whole cultures. Every culture was established in triplicate and visualized under an inverted microscope (×400 magnification). b) Semiquantification of the tube formation index in HUVEC after incubation with 8 μM Pt. There is a drastic reduction in the number of capillary-like structures formed upon incubation with this compound. Bars correspond to the percentage of the number of tubule-like structures comparatively to control. Statistical differences between groups were evaluated by one-way analysis of variance (ANOVA test) followed by the Dunnet test (mean ± SD; *p < 0.01 vs. Control).

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