Figure 5From: Petiveria alliacea extracts uses multiple mechanisms to inhibit growth of human and mouse tumoral cells Effect of Petiveria alliacea F4 fraction on cell cycle. A. A375 cells treated with ethanol (0.2%), or F4 fraction (31.2 μg/ml) or vincristine (0.1 μg/ml) for 24 h, were permeabilized, stained with propidium iodide (PI) 50 μg/ml and analyzed through flow cytometry (FACScalibur CellQuest software program) (Becton Dickinson). Bars represent relative percentage of cell-cycle distribution ± SEM and represent three independent performed experiments. ***p < 0.001 versus control (ethanol 0.2%; Unpaired Student's t-test). B. A375 cells treated with ethanol (0.2%) or F4 fraction (31.2 μg/ml) or vincristine 0.1 μg/ml for 12, 18, 24 and 48 h, were permeabilized, stained with PI 50 μg/ml and analyzed through flow cytometry (FACScalibur CellQuest software program) (Becton Dickinson). Histograms represent relative cell DNA content representing two independent experiments. C. A375 cells treated with ethanol (0.2%) or F4 fraction(62.5, 31.2, 15.6 μg/ml) or vincristine (0.1 μg/ml) for 48 h, were permeabilized, stained with PI 50 μg/ml and analyzed through flow cytometry (FACScalibur CellQuest software program) (Becton Dickinson). Histograms represent relative cell DNA content representing two independent experiments.Back to article page