Figure 3From: Induction of apoptosis of human primary osteoclasts treated with extracts from the medicinal plant Emblica officinalis A, B. Detection of apoptosis by TUNEL assay in human primary osteoclasts. The presence of apoptotic OCs after treatment with 0.5, 5, 50 μg/ml of Emblica officinalis extract for 60 hours is shown in panel A. Brown color reaction indicates cells that underwent apoptosis. Quantitative results of the experiment shown in panel A are depicted in panel B (the data reported represent the average ± SD of six independent determinations). C. Immunocytochemical analysis of FAS receptor expression levels in human primary osteoclasts subjected to the same experimental conditions reported in panel A. (-): control cells. Cells were photographed at the 20 × magnification.Back to article page