Figure 1

TRAP staining analysis of human primary osteoclasts obtained after 14 days of culture in presence of 0.5, 5, and 50 μg/ml of Emblica officinalis extracts, as indicated (A); the same percentage of multinucleated TRAP-positive cells was obtained when mature osteoclasts were grown for 60 hours with the same amount of Emblica officinalis extracts (B). Cells were photographed at the 20 × magnification. In the lower part of the panel data from five determinations are presented (average ± SD).