Figure 8

Effects of Kihi-to on Aβ(25–35)-induced increases of calpain and decreases in calpastatin. Aβ(25–35) (25 nmol) was injected into the right lateral ventricle of mice. From ten days after the injection, mice were administered vehicle (V, water by p.o.; DMSO by i.v.) or Kihi-to (K, 100 mg/kg B.W., p.o.) for 3 days. The control mice (C) were injected with a reverse peptide, Aβ(35–25) and then administered vehicle. After the novel object recognition test (Figure 2), brain slices were immunostained with μ-calpain (A) or calpastatin (B) antibody. Calpain-positive and calpastatin-positive areas were quantified in the stratum lucidum in CA3, and the parietal, perirhinal and frontal cortex. *p < 0.05 vs. Veh. n = 3. (one-way ANOVA followed by Holm-Sidak post hoc test).