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Figure 2 | BMC Complementary and Alternative Medicine

Figure 2

From: Novel phytoandrogens and lipidic augmenters from Eucommia ulmoides

Figure 2

Schematics of research process flow. E. ulmoides cortex was macerated and crude extracts obtained using solvents. These solvents were dichloromethane (DCM), ethyl acetate (EA), ethanol (EtOH) and water (H2O). The crude extracts were subjected to steroidogenic reporter gene and binding assays. Bioactive fractions were subsequently obtained through bioassay-guided fractionations using column chromatography, thin layer chromatography and high performance liquid chromatography (HPLC). Pharmacologically active phytocompounds purified from the bioactive fractions were identified through spectroscopic and nuclear magnetic resonance (NMR) studies. Confirmatory reporter gene assays were then carried out using pure compounds to reproduce steroidogenic effects.

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