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Table 4 Dosage effect of MAK-4 and MAK-5 on the growth of transformed Rat 6 cells

From: MAK-4 and -5 supplemented diet inhibits liver carcinogenesis in mice

Treatment Cells (× 106/60 mm plate) ± SD % of survival
  Rat 6 R6/T24* Rat 6 R6/T24
None (control) 0.94 ± 0.04 1.32 ± 0.04 100 100
MAK-4:     
200 μg/ml 1.24 ± 0.12 1.52 ± 0.05 138 141
500 μg/ml 0.94 ± 0.08 1.27 ± 0.06 112 115
1000 μg/ml 0.82 ± 0.09 1.29 ± 0.07 91 119
MAK-5:     
200 μg/ml 1.57 ± 0.09 1.77 ± 0.09 119 164
500 μg/ml 0.84 ± 0.08 1.07 ± 0.04 84 101
1000 μg/ml 0.58 ± 0.06 0.73 ± 0.01 66 79
  1. R6/T24 is a permanent cell line transformed by an activated human c-H-ras oncogene. Both normal and H-ras transformed Rat 6 cells were seeded in triplicate at 104 cells per 60 mm plate in D10CS. Next day, cultured were switched to D5FCS, or FCS plus herbal extracts at designated dosages for 5 days. At the end of the treatment, cells were trypsinized and counted by Coulter Counter. Cytotoxicity was expressed as percent of cell survival compared to the untreated control.